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KMID : 0376219840210010085
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Chonnam Medical Journal
1984 Volume.21 No. 1 p.85 ~ p.93
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The Role of Ascorbic Acid in Hemoglobin Breakdown in Vitro
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Abstract
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The oxidative breakdown of hemoglobin by ascorbic acid has been thought to proceed autocatalytically, but there are still some uncertainties about the mechanism. In the present study, the role of ascorbic acid in this reaction has been investigated by examining the effects of peroxidase, catalase, hydrogen peroxide, superoxide radical, and free hydroxy radical scavengers on the methemoglobin breakdown by ascorbic acid. Peroxidase, but not catalase, almost completely prevented the hemoglobin breakdown and diminished the ascorbate consumption in the reaction. Hydrogen peroxide, which was generated by glucose oxidase system, and superoxide radical did not directly oxidize hemoglobin by themselves, but when present together with ascorbate, they enhanced the degradative action of asccorbate. Scavengers of hydroxy radical such as sodium benozoate, ethanol and mannitol had no effect on the action of ascorbic acid.
Based on these results, it is proposed that an active peroxy intermediate of ascorbic acid is generated during the hemoglobin breakdown by ascorbic acid and that this ascorbic peroxy intermediate directly oxidzes hemoglobin to choleglobin.
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KEYWORD
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